Tournament

From DBZ
Goku

Vegeta

Gohan

Piccolo

Gotenks

Tien Shinhan

From Yu Yu Hakushow
Yusuke Urameshi

Karama

Kazuma Kuwabara

Hiei

From Naruto
Naruto Uzamaki

Kakashi Hatake

Sauske Uchiha

Ruroni Kenshin
Himura Kenshin

Sanosuke Sagara

From Samurai Jack
Samurai Jack

From One Piece
Luffy

Roronoa Zoro

Sanji

Portgas D.Ace (Non-Competitor)

From Megas XLR
Coop with Megas

Avatar
Aang (After Sozin's Commit)

Zuko (After Sozin's Commit)

From Ben10 series
Ben (Ultimate Alien) with Diamondhead, Humungosaur, and Swampfire

Kevin Levin (Ultimate Alien)

From DC Universe
Batman

Superman

Flash

Captain Marvel

NightWing

From Marvel
Spiderman

Hulk

Wolverine

Captain America

Thor

Black Widow

From TMNT

Raphael

Michangelo

Leanardo

Donatello

Miyamoto Usagi

Games
Note: I know absolutly nothing about God Of War, so those characters will not appears.

From Mortal Kombat
Liu Kang

Kung Lao

Goro

Raiden

Fujin

From Street Fighter
Ken

Ryu

Rashid

Chun-Li

Matches

Ken vs Goro

Kakashi vs Captain America